Differential Expression Of Indole Alkaloid Genes In The Medicinal Plant Catharanthus Roseus
Indole alkaloids such as vinblastine and vincristine in the medicinal plant Catharanthus roseus are two of the most widely used compounds in cancer chemotherapy against various leukemia, Hodgkin's disease and solid tumors. In this study, transcriptome data of C. roseus across organs was generated via Next-Generation Sequencing (NGS) technique and stored in NCBI. RNA-Seq data was collected and de-novo assembled. Resulted differentially expressed (DE) transcripts were analyzed using bioinformatics software to detect the localization of TIA pathway in different of plant organs tissues (flower, stem, root, hairy roots, mature leaf and immature leaf). The findings showed that DE transcripts related to vincristine and vinblastine accumulation are highly expressed in roots, flowers, and mature leaves tissues. Hopefully, the outcomes will provide a new insight into genes and pathways involved in regulating indole alkaloids compounds. In addition, the isolated genes from this study could facilitate in vitro overproduction of these compounds via metabolic engineering approaches.