Paper Title
Isolation And Identification Of Antioxidant Compounds From Piper Methysticum Root

Abstract
Bioassay-guided fractionation of the ethyl acetate extract of kava root resulted in the isolation and purification of six fractions using column chromatography under direction of DPPH and ABTS assays. They included three flavonoids: 5-hydroxy-4’,7-dimethoxyflavanone (C1), matteucinol (C2) and 5,6-dihydroxy-7-methoxyflavone (C3) and three kavalactones: 5,6-dehydrokawain (DK) and 5,6-dihydromethysticin (C4), DK (C5), and yangonin (C6). The evaluation of free radical scavenging activity of the isolated substances by DPPH and ABTS assays revealed that C1 (IC50: ABTS = 203.5 µg/ml), C2 (IC50: ABTS = 173.1 µg/ml), C3 (IC50: ABTS = 75.2; DPPH = 75.1 µg/ml), and C4 (IC50: ABTS = 181.7; DPPH = 1210.2 µg/ml) have significant antioxidant properties. This study was the first to purify and identify the flavonoid C3 in kava root and report on its antioxidant potential. Index Terms - Piper methysticum L., antioxidant activity, antibacterial activity, flavonoid, kavalactone.