Paper Title
Production and Characterization of Exopolymeric Substances with Emulsifying Activity by the Marine Bacterium Kocuria Rosea

Abstract
An extracellular polymeric substance (EPS) producing marine bacteria, identified and characterized asKocuria rosea strain 23Bbased on 16S rRNA sequencing and phylogenetic analysis(99 % similarity), was isolated fromCoast of Jalisco (Mexico). Glucose (1 %) was the most efficient carbon source for growth and EPS production. The highest EPS production (6 g/L) was yielded in fermenter culture at 250 rpm after 48 h of incubation.The EPS produced was capable of stabilizing water emulsions with kerosene and hexane.Results showed surface tension reduction and emulsion activity valuesfrom 58.80 ± 1.86 mN/m and 40 ± 0.34 to 52.32 ± 0.07%, respectively.Analysis of extracted EPS using Fourier transform infrared spectroscopyrevealed “finger sprint” to polysaccharides and protein. Keywords - Marine bacteria, Kocuria rosea, extracellular polymeric substances, emulsifying activity