A Novel Headspace Soild-Phase Microextraction Of Triclosan With Sublimated Camphor Sorbent In Environmental Samples For Gc-Ecd Analysis
A sublimate camphor sorbent was prepared for solid phase microextraction (SPME) of triclosan in environmental samples prior to gas chromatography-micro-electron capture detection (GC-μECD). The sublimated camphor was coated onto a fused silica fiber as one-time-used SPME sorbent.
Parameters affecting the extraction efficiencies included the coating (solvent for camphor and the temperature), extraction (sample pH, stirring rate, time, and salting out effect). The maximum extraction efficiency was obtained under the conditions as follows: a fused silica fiber was dipped into camphor solution for 10 sec ( at 50℃). Then the coated fiber was moved into the 20-mL vial with 5 mL sample solution for a 40-min headspace extraction with stir rate 800 rpm (at 60℃). After extraction, the fiber was injected into GC-ECD directly. The linear range was 0.025-20 μgL-1 with coefficient of determination of 0.9958. Detection limit of triclosan was 6.9 ngL-1 with relative standard deviation (RSD) of 5.4% and quantitation limit was 23.1 ngL-1. The relative recoveries of spiked samples were 84.7 – 101.8% with RSDs of 2.9 – 6.8% in real samples. The proposed sublimation sorbent obtained excellent enrichment factor (848) and provides a simple, rapid, sensitive, and eco-friendly sample preparation method.
Keywords - SPME, Camphor, Triclosan, GC-ECD