Investigation of Polymorphism and Mutation in the Exon1 Region of MBL2 Gene at Patients with COPD
Introduction and objectives: Obstructive pulmonary disease (COPD) is characterized by chronic airway limitation. The most common causes of COPD are smoking and respiratory infections. Mannose-binding lectin (MBL) belongs to the family of collectins and plays a role in the complement activation of the innate immunity through the lectin pathway.
Materials and Methods: In this study, following DNA extraction in 59 patients with COPD, PCR was used to amplify the exon 1 of MBL2 gene. The PCR products were later sequenced and BLAST search was performed on them. The results were analyzed using SPSS.
Results: The results of nonparametric statistics showed that there was a significant relationship only between nucleotides 304 deletion mutation (T) of the gene MBL2 exon 1 and there was not a significant correlation between the response to treatment and remaining polymorphisms and mutations and in the studied subjects.
Keywords – Mannose Binding Lectin (MBL), Chronic Obstructive Pulmonary Disease (COPD), Polymerase Chain Reaction (PCR), Polymorphism