Paper Title
Isolation of A New Lysinibacillus Strain Producing Antibacterial Proteins from Rotten Citrus
Abstract
To investigate a reusable method of citrus peels and low-value citrus fruits, microbes from rotten citrus were analyzed. A bacterium with antibacterial activity was isolated and further analyzed. Phylogenetic analysis with 16S rDNA from the bacterium was reveled to new Lysinibacillus strain. The Lysinibacillus strain has strong antibacterial activity against Gram(+) bacteria such as Staphylococcus aureus, Lysinibacillus sphaericus and Pichia scaptomyzae but not Gram(-) bacteria such as Escherichia coli and Enterobacter aerogenes. The antibacterial activity was most increased on the culture conditions of 24℃, 7 days in the YPD broth and the activity was disappeared by treatment of heat or trypsin. These results indicated that the new Lysinibacillus strain produce antibacterial proteins against Gram(+) bacteria.
Keywords - Lysinibacillus, Antibacterial Proteins, Rotten Citrus