Paper Title
Why Zat12 Is Crucial In Stress Tolerance In Wheat Transgenics?

Abstract
Zat12 encodes a zinc-finger transcription protein and play a significant role in modulating defense and acclimation responses in plants. In order to make the wheat crop tolerant to abiotic stress conditions, Zat12 transformed wheat lines were produced in the background of PBW 621 by agrobacterium mediated gene gun method. To gain indepth knowledge of Zat12 related biochemical activities in stress tolerance, expression of Zat12 gene was analyzed through RT-PCR. Activities of antioxidative enzymes (SOD, POD, GR, GST) alongwith proline and ascorbate content have been studied under non stress and stressed conditions (heat and cold) in transformed lines (Z-8-12 1A, Z-8-12 1B, Z-8-19 and Z-15-10) and wheat cultivars (PBW 621, PBW 550 and HD 3086). It was observed that Zat12 transformed lines led to stimulation of antioxidative defence system, overexpression of antioxidative enzymes was reported under temperature stress in Zat12 transformed wheat lines. Keywords - transcription factor, proline, ascorbate, superoxide dismutase (SOD), peroxidase (POD), glutathione reductase (GR), glutathione-s-transferase (GST), antioxidative enzymes