Paper Title
A Design of EXA Design of Experiment (DOE) Approach to Investigate Synergy Effects Among Natural Products

Abstract
In order to find a significant synergy effects among the combinations of a number of natural products, many researchers have been investigated possible combinations of existing natural products. Because of current foods and lifestyles, an obesity issue has considered as a significant problem and research focus. However, in much literature, many existing approaches may not demonstrate systemic designed experiments and may not consider statistical correlations. The main purposes of this research is to propose a design of experiment (DoE) method and to demonstrate its associated statistical analysis approaches. First, we focused on the inhibition of adipocyte differentiation among a number of selected natural products (i.e., Astragaloside I, Ginsenoside Rd, 3,4-Dicaffeoylquinic acid, and Corilagin) based on existing literature. Second, in order to investigate synergy effects including correlations among a number of natural products, we then proposed a simplex centroid design for this experiment. Third, based on the results of the DoE method and its associated analysis, we further investigated the optimal combinations among the selected natural products. Finally, the experiment results (i.e., DoE, statistical correlation, and optimization) provided a significant possible combination of the selected natural products. Keywords - Mixture centroid design, Desirability function, Natural product,Hedgehog signaling,Astragaloside I, Ginsenoside Rd, 3,4-Dicaffeoylquinic acid, Corilagin