Paper Title
Insights Of Toxicity Induced By Zinc Oxide (ZNO) Nanoparticle Using Systems Network Approach

Abstract
Zinc oxide nanoparticles (ZnO-NPs) are widely used in various industries like consumer products, paint, and pharmaceutical preparations. ZnO-NPs induce reactive oxygen species (ROS) formation in a human cell, leading to the generation of cytotoxicity, genotoxicity, and carcinogenicity. In the present study, we analyzed the ZnO-NP interaction with proteins through various computational system network approaches for the screening of potential target, which may play an effective strategy for the understanding of toxicity mechanism of ZnO-NP within the biological system. We have constructed the ZnO-NP and proteins interaction network map by using computational approaches and experimental databases. After topological, network and directional mapping analysis was performed to identify a set of candidate that may play important roles in toxicity mechanism of ZnO-NP within the system. IL6 is the most potential target that we was found in our system analysis and which may be act as a hub node for the interaction network and also be a promising target for further experimental validation. After screening of potential protein from the system network, molecular docking methodologies were performed using Auto Dock 4.2.6. software. We have observed binding affinity of ZnO-NP with IL6 protein is -5.71 Kcal/Mol and also screen the interaction pattern of amino acid residues which play important role in binding affinity again the ZnO-NP. Keywords - Zinc oxide nanoparticles (ZnO-NPs), Molecular docking, AutoDock, Nanoparticle-Protein Interaction.