Investigating The Multidrug Resistance Of MRSA And The Genes Involved
MRSA, which stands for Methicillin Resistant Staphylococcus Aureus, is a strain of the bacterium Staphylococcus Aureus which is a coagulase positive gram positive bacterium. It is very dangerous, resistant to drugs, and can even cause serious, life-threatening problems. This study is focused on identifying the gene that is related to drug resistance. For the investigation of the multidrug resistance of MRSA, a sample of MRSA was obtained from a local hospital. The DNA from MRSA was extracted and mixed with E. coli for transformation of the host bacteria. The resistance of the transformed E. coli was tested in different concentrations of ampicillin, gentamycin, kanamycin, and tetracycline. To investigate the genes that were involved in the multidrug resistance, I first prepared the transformed E. coli via mini prep, and then ran the E. coli through PCR. Finally, the method of gel electrophoresis was used to identify the genes involved in drug resistance. According to the results, both MRSA and the genetically transformed E. coli show drug resistance to some antibiotics. It can be interpreted that the DNA that codes for antibiotic resistance was successfully transported into the E. coli that survived the antibiotics. The DNA was amplified in both the drug resistant MRSA and E. coli through PCR. Since the section of DNA that codes for the multidrug resistance is most likely shared in all the bacterium that survived the antibiotics, the DNA involved in drug resistance can be identified through electrophoresis.
Index Terms- ampicillin, gentamycin, kanamycin, MRSA (Methicillin Resistant Staphylococcus Aureus), multidrug resistance, tetracycline