Paper Title
Evaluation of Staphylococcal Enterotoxin Beffect on The Endoplasmic Reticulum Mediated Apoptosis

Abstract
Staphylococcal enterotoxin B is known as a potent superantigen that is able to attach to MHC II molecules located on the surface of antigen presenting cells and induce a robust immune response via proliferation and activation of T cells. Numerous previous studies used this superantigen as an adjuvant and immune enhancer for cancer therapy. Their finding showed that SEB had an anti-tumor effects. Furthermore, a modeling analysis reported the influence of SEB on the FAS- mediated apoptosis. Here, we aimed to survey the apoptotic effects of SEB on the breast cancer cell line. To identify the cytotoxic and apoptotic impacts of SEB, MDA MB-231 cells were treated with 5, 25, 50 and 100 μg/ ml SEB and then, the expression ratio of GRP-78, IRE-1 and CHOP genes involved in apoptosis was assessed by Real time –PCR.GRP-78 level was significantly attenuated after 24 h (P <0.05) but the expression ratio of CHOPand IRE-1 had no difference compared with control group. SEB has apoptotic properties against tumor cells and effect on factors involved in endoplasmic reticulum mediated apoptosis. Keywords- Staphylococcal enterotoxin B, cell death, superantigen, breast cancer