Bioremediation Potential of Indigenous Microorganisms in Soil Contaminated with Diesel Fuel
The objective of this research was to investigate the effect of different concentrations of diesel fuel in soil on the number and activity of the microorganisms, in the presence or without plants root, in order to evaluate their effectiveness as potential bioremediation agents. The cuttings of poplar clone Populus x euramericana cl.129/81 were cultivated in soil with different levels of pollution. The variants of the experiment were the following: soil with 0, 5 and 20 g of diesel / kg; plant planted in soil with 0, 5 and 20 g of diesel /kg; control pots with and without plant. Microbiological analyses were made in laboratory for microbiology on Department of Crop Science, Faculty of Agriculture in Novi Sad. On average, the largest number of bacteria and actinomycetes were determined after 90 days and for fungi after 30 days. Dehydrogenase activity grew during the time in variants with the plant, while in other falling. The presence of plants root had a good influence on surviving and activity of microorganisms in the contaminated soil. The number of the investigated groups of microorganisms and dehydrogenase activity in presence of plant increased in variants with higher concentration of diesel fuel in comparison with the control. In variants without plant, number and dehydrogenase activity of microorganisms increased in variants with lower concentration of diesel fuel.
Key words - Microorganisms, dehydrogenase activity, plant, diesel fuel, bioremediation