Development of Short Amplicon Length PCR Assay for the Detection of Porcine DNA in Gelatin Containingcommercial Food Products-Halal-Kosher Authentication
Halal gelatin source authentication is significant for consumer satisfaction and religious faith. Mislabelling of commercial gelatin based products is a common fraud, particularly for pharmaceutical capsule, dairy and chocolate products, because of the limitations of halal gelatin production. Up-to-date development in molecular biology allowed researcher for setting up efficient and universal omics to unambiguously detecting the exact origin of gelatin even after packaging to ensure their traceability. In this context, molecular discrimination of commercial products via Polymerase Chain Reaction (PCR)holds the greatest promise in terms of amplifying minute levels of DNA targets from a mixture of DNA molecules. Although, development of short amplicon length biomarker for porcine and extraction of DNA from highly processed gelatin based food products is a crucial step for molecular based analytical approach. Here, we described the step by step procedure for development of short amplicon length PCR assay for the detection and differentiation of porcine DNA in processed food products (such as gummy candy, chewing gum, dairy products, ice-cream and so on ).
Key words - Gelatin; Short length biomarker; Authentication;PCR assay; Processed food product