Direct Rhizogenesis, Phytochemical Analysis and Antimicrobial Activity of Bryonia Laciniosa
Efficient rhizogensis was established for Bryonia laciniosa using leaf derived calli. The maximum morphogenic callus induction rate (80%) was observed from leaf explant by culturing in MS medium supplemented with 2.0 mg/l IAA + 2.0mg/l IBA. Calli size and fresh weight increased substancially through subculturing. The highest percentage of root induction (70%) and highest mean number of roots (12.33) per culture were obtained with 2.0 mg/l IAA + 2.0 mg/l IBA. Phytochemicals like alkaloids, tannins, flavonoids, saponins and glycosides were also found present. The study indicates the presence of nutritional and chemical components that are beneficial in addition to the numerous medicinal values of the plant. Antibacterial effect of Bryonia laciniosa was evaluated on bacterial strains like Bacillus cereus, Staphylococcus aureus, Pesudomonas Enterobacter aerogenes, Escherichia coli and Klebsiella pneumoniae. The solvents used for the extraction of plants were water and methanol. The in vitro antibacterial activity was performed by agar disc diffusion. The most susceptible bacteria was K. pneumoniae. The extracts of callus and plant material could not inhibit any of the bacterial strains investigated.
Keywords - Rhizogenesis, Antimicrobial, Bryonia Laciniosa, Phytochemical Analysis