Paper Title
Identification And Characterization Of A Novel Antimicrobial Peptide Fk11 And Its Potential Activity As Therapeutics Against Bacterial Infection

Abstract
Numerous antimicrobial peptides (AMPs) from a natural substance have been identified, isolated and characterized. We have identified and researched on a novel antimicrobial peptide, FK11 which was isolated from pine needles. Originally, FK11 has four amino acids in the C-terminal. However, it couldn’t be identified of the four amino acids that make up it. Necessarily, FK11 was synthesized for identified amino acids and it was active as an antimicrobial peptide. Unlike other peptides, FK11 is derived from crude plant and a very short antimicrobial peptide consisting of only 11 amino acid sequences. It is an antimicrobial peptide including arginine and lysine. Therefore, FK11 shows strong antimicrobial activity against gram-negative and gram-positive bacteria but also exhibits no cytotoxicity toward mammalian cells. FK11 appeared to act on the bacterial membrane. Additionally, binding of lipopolysaccharide results in inhibition of pro-inflammatory cytokine secretion. Then, FK11 has anti-inflammatory activity in RAW 264.7 cells exposed to LPS of gram-negative bacteria. This study suggests that FK11 acts as host defense molecules that exerts antimicrobial effects by targeting the lipopolysaccharide of gram-negative bacteria. Moreover, it has been reduced production of pro-inflammatory mediators and correspondingly will treat inflammation diseases. FK11 will further research how to inhibit inflammation in the future, related to NF- κB pathway. Except that, FK11 will have a lot of research work to be done. Keywords - Antimicrobial peptide, Arginine, Lysine, Lipopolysaccharide, Anti-inflammatory activity