Paper Title
Isolation Of Novel Antifungal Peptieds From Bacillus Amyloliquefaciens Pt14 Against Fusarium Solani And Fusarium Oxysporum

Abstract
Bacillus species have recently drawn attention due to their potential use in the biological control of fungal diseases. This paper reports on the antifungal activity of novel peptides isolated from Bacillus amyloliquefaciens PT14. Reverse-phase high-performance liquid chromatography revealed that B. amyloliquefaciens PT14 produces five peptides (BAPT1, 2, 3, 4A, and 4B) that exhibit antifungal activity but are inactive against bacterial strains. In particular, BAPT3 and BAPT4A showed broad-spectrum antifungal activity against Fusarium solani and Fusarium oxysporum. The BAPT4A N-terminal amino acid sequence was identified through Edman degradation, and a BLAST homology analysis showed it not to be identical to any other protein or peptide. BAPT4A displayed strong fungicidal activity with minimal inhibitory concentrations of 3.12 mg/L (F. solani) and 6.25 mg/L (F. oxysporum), inducing severe morphological deformation in the conidia and hyphae. On the other hand, BAPT4A had no detectable hemolytic activity. This suggests BAPT4A has the potential to serve as an antifungal agent in clinical therapeutic and crop-protection applications. Keywords - Bacillus amyloliquefaciens, Antifungal peptide, Fusarium solani, Fusarium oxysporum, crop-protection application