Bioremediation by Single Culture of Pseudomonas Putida in Petroleum Contaminated Soil
Land contamination by petroleum exploration, production and discharge of waste causing serious damage to the ecosystem of the environment, human and animal. Biodegradation (bioremediation) processes have difficulty focused due to the complexity of the hydrocarbons that was adsorbed by the soil. The objective of this research was to determine the efficiency of bioremediation as a function of bacterial concentration. A method that was developed in bioremediation of petroleum-contaminated soil in addition to in-situ bioremediation was the ex-situ bioremediation with slurry phase bioreactor. The mixture of soil and water were fed in the bioreactor. The aerobic bacteria used in biodegradation of benzene, toluene, and xylene (BTX) process were Pseudomonas putida. The variables that would be measured were BTX concentration; bacterial population and operating conditions (temperature, pH and dissolved oxygen). Residues of petroleum hydrocarbon (BTX) were measured by gas chromatography method. Process was identified during 56 days in 3 bioreactors in parallel. Reactor was added by 12,5% (v/v) (A), 15% (v/v) (B)and 17,5% (v/v) (C) Pseudomonas putida bacteria. The bioreactors were agitated and aerated during bioremediation process. After 56 days, this research resulted in total degradation of BTX in reactor A reached 91,0400%; reactor B 91,1489%; and reactor C 97.6726%.
Keywords- Pseudomonas putida; bioremediation; Benzene; Toluene; Xylene; slurry bioreactor