Paper Title
Cultivation and Harvesting of Microalgae in Photobioreactor for Biodiesel Productionand Simultaneous Nutrient Removal

Microalgae are recognized as a potential source of sustainable biomass feedstock for biofuel production and are capable of proliferating under versatile environmental conditions. In the current study, Chlorella vulgaris and Scenedesmus obliquus were cultivated in a small scale vertical flat-plate photobioreactor (PBR) supplemented with municipal wastewater in order to achieve simultaneous wastewater treatment and biomass production for biofuel generation. Microalgal growth and nutrient removal including total nitrogen (TN), total phosphorus (TP), total inorganic carbon (TIC) and trace elements (Ca2+, Na+, Mg2+ and Zn2+) were monitored during microalgae cultivation. C. vulgaris and S. obliquus showed optimal specific growth rates (ľopt) of 1.39 and 1.41 day-1, respectively, and the TN and TP were completely removed (>99%) from the wastewater within 8 days. Microalgal biomass in the PBR was harvested using a natural flocculant produced from Moringa oleifera seeds. The harvesting efficiency of M. oleiferawas 81% for C. vulgaris and92% for S. obliquus. The amounts of saturated, mono-unsaturated, and poly-unsaturated fatty acids in the harvested biomass accounted for 18.66, 71.61 and 9.75% for C. vulgaris and 28.67, 57.14 and 11.15% for S. obliquus, respectively. The accumulated fatty acids were suitable to produce high quality biodiesel with characteristics equivalent to crop seeds oil- derived biodiesel. This study demonstrates the potential of microalgae-based biodiesel production through the coupling of advanced wastewater treatment with microalgae cultivation for low-cost biomass production in a PBR.