Optimization of Ultrasonic-Assisted Extraction of Antioxidant Compounds from Elaegnus Angustifolia Using Response Surface Methodology
In this investigation optimization of flavonoid extraction process frome methanolic (80%) extract (v/v) of elaegnus angustifolia through Response Surface Methodology by according to Lamaison and Carnat is performrd for optimizing the optimizing the process three factor (time, temperature and solution to solid ratio) were investigated each factor was repeated three times (5,20,35 min), temperature (30,45,60 ° C), and solution to solid ratio (20,35,50 ml/g). The experiment was conducted based on Box-Behnken design with three factor and three levels comprising a total of 17 tests. During optimization tests in an experiment with extraction conditions as (time:31/13 min, temperature:46/79 ∙C and solution to solid ratio: 32/71 ml/g) the higest flavonoid 32/21 mg quercetin (standard of Flavonoid compound) per g of dry material compound was recorded. In addition to Flavonoid test, beta-carotene / linoleic test was also carried out for investigating antioxidant activity. In beta-carotene / linoleic test methanolic extract with 24/65 mg E.Q/g had the highest antioxidant activity.Considering the results and graphs temperature was indetified as the most effective factor.
Keywords- Elaegnus Angustifolia, Flavonoids, Antioxidant, Extraction, Ultrasound