Purification Of Siderophores From Pineapple (Ananas Comosus)
Siderophores are important natural iron chelator. They are used as one of the commercial drugs for human blood-iron excess disease (Thalassemia) treatment. This chelating compounds have been founded only in some graminaceous plants, fungi and most rhizospherical micro-organisms under condition of iron-deficiency. Nevertheless, primary tests of aqueous extracts from each part, e.g., top, slip or leaf of pineapple (Ananas comosus) showed positive results of iron chelating ability with chrome azurol s (CAS) solution test. Isolation and purification of the active components revealed that its siderophores are hydroxamate type mixed with catecholate. This is the first report on siderophore in the bromeliads which is non-gramineceous plantís family.
Keywords- Siderophore; Iron chelator; Pineapple (Ananas comosus); Waste; Extraction; Isolation; Purification; Partial identification; Chrome azurol s (CAS)