Biochemistrical and Environmental Applications: Utilization of Laccase Enzyme as Ioremidiation in Petroleum Industry Waste by Reducing Pah (Polyaromatic Hydrocarbon)
Laccase (benzendiol: oxygen oxidoreductase, EC 220.127.116.11) is an extracellular enzyme using oxygen compounds to run the oxidation reaction of various aromatic and nonaromatic compounds. Laccase enzyme are found in fungi, such as the Basidiomycetes, Ascomycetes, and Deutromycetes. Other sources of this enzyme are plants, insects, and bacteria, especially prokaryotes. Acer pseudoplatanus, Pinus taeda, Aesculus parviflora, and Populus eruamericana are the kinds of plants that are capable of producing the laccase enzyme. There are a lot of application of laccase enzyme, such as laccase in the paper industry, laccase in the textile industry, Medical and Personal Care of laccase, laccase in the food industry, Biosensors and Diagnostic Applications of laccase and for bioremediationThis research focus on aplication of laccase enzim as bioremidiation in waste of petroleum industry with reducted the PAHs (Poliaromatic Hydrocarbon) on it. The purpose of this study was to determine the utilization of laccase enzyme as bioremidiation in petroleum industry waste by reducing PAHs (Poliaromatic Hydrocarbon). The study was conducted with a sample media preparation, analysis of oil content (gravimetric), pH analysis, and analysis of the number of microbes TPC (Total Plate Count). Parameters were observed to determine the optimal conditions include: temperature, pH, water content, TPC (Total Plate Count) and degradation of pencentage of TPH (Total Petroleum Hydrocarbon). This review help to understand about propertise, aplication and optimalization laccase enzyme as bioremidiation in petroleum industry waste for its biochemistrical and environmental applications.
Keywords- laccase enzyme, PAH (Poliaromatic Hydrocarbon), bioremediation.