Paper Title
FABRICATION AND DRUG RELEASE PERFORMANCE OF SUPRAMOLECULAR NANOCONTAINERS OF CIPROFLOXACIN/IBUPROFEN/CYCLODEXTRIN TO IMPROVE HEALING IN WOUND DRESSING APPLICATION

Abstract
Abstract - Modern wound dressings such as electro spun mattresses provide a controlled environment of moisture, relieve infection, relief of pain, and prevent dehydration at wound sites which results to faster wound healing effect. The simultaneous use of drugs of ciprofloxacin (CIP) and ibuprofen (IBU) in wound dressing is of interest to reduce infection and pain due to ability of CIP for terminating the bacterial growth and ability of IBU to relieve pain and inflammation. However, there is a need to use cyclodextrins to improve the side effects of Ibuprofen usage due to its poor bioavailability and gastrointestinal toxicity. Cyclodextrins are used as a host compound to form complexes with CIP and IBU as guests. The complex product is then loaded by electrospinning for targeted applications. In the paper we elaborate fabrication of supramolecular nanocontainers of CIP/IBU/cyclodextrins which were prepared via formation of inclusion complex between ciprofloxacin, ibuprofen and cyclodextrins (a-cyclodextrin and b-cyclodextrin) at various conditions (at room temperature at 25°C and under sonic energy). The formation of inclusion complex between CIP, IBU and cyclodextrins and intactness of drugs in the complex were confirmed by Differential Scanning Calorimetry (DSC), X-Ray Diffraction Analysis (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Nuclear Magnetic Resonance (HNMR), transmission electron microscopy (TEM) and scanning electron microscopy (SEM) study. The drug release of CIP and IBU from polycaprolactone nanofiber mat at pH=7.2 was investigated in laboratory conditions where the drugs control release was confirmed by UV–visible spectrophotometry. The release of antibacterial drug of ciprofloxacin indicated the healing potential of wound dressing by showing excellent antibacterial properties against gram-positive and gram-negative bacteria. Electro-spun nanofibers of polycaprolactone containing CIP/IBU/cyclodextrins is a promising method for controlled drugs release for a multi-tasking modern wound dressing. Keywords - Bioavailability, Ciprofloxacin, Electrospinning, Ibuprofen, Inclusion Complex, Solubility, Α-Cyclodextrin, Β- Cyclodextrin.