Paper Title
Preparation and Characterization of Chitosantobramycin Nanoparticles In Combination with Zinc Oxide Nanoparticles

Abstract
Herein we describe the preparation, characterization and the antibacterial effect of Tobramycin loaded in chitosan nanoparticles in combination with metallic (Zinc oxide) nanoparticles. Chitosan-tobramycin nanoparticles were prepared by the ionic gelation method using TPP as a cross-linker. The NPs size, zeta potential, polydespersity, entrapment efficacy, morphology, antibacterial activity and the drug release in vitro were investigated. Zinc oxide nanoparticles (ZnO NPs) were prepared by the hydrothermal method. Then, the ZnO NPs were physically mixed with chitosan-tobramycin nanoparticles and the combination was analyzed by X-ray powder diffraction (XRD), Fourier transform infrared spectrometer (FTIR). The antibacterial activity of the Zinc oxide nanoparticles, Chitosan-tobramycin nanoparticles and their mixture against P. aureginosa. S.aureus and E.coli was studied. Results showed that the chitosan nanoparticles controlled the release of the antibiotic and had good antibacterial activity. Zinc oxide nanoparticles had no antibacterial activity and the addition of Zinc oxide nanoparticles had no effect on the antibactericidal effect as Chitosan-tobramycin nanoparticles. Keywords - Chitosan-Tobramycin Nanoparticles, Zinc Oxide Nanoparticles, Antibactericidal Effect