Paper Title
The Effects Of Copper Oxide Nanoparticles On The Alt And AST Enzymes In Common Carp (Cyprinus Carpio)

Abstract
As a result of the development of nanotechnology, there are increasing concerns about the potential dangers of chemical release containing nanoparticles on the environment. The aim of this study was to evaluate the effect of copper oxide nanoparticles (CuO NPs) on AST and ALT in the blood serum of common carp. Six groups of carp (Cyprinus carpio) were studied with a weight of 352 g involved in the control group and 5 groups exposed to various concentrations of copper nanoparticles (5, 10, 50, 100 and 150μg/l). Bleeding was performed from the caudal fish treatments at 24, 48, 72 and 96 h and prior exposure to nanoparticles (base). An auto-analyzer was used to measure the serum concentrations of the enzymes ALT and AST. Statistical analysis showed that fish exposed to nanoparticles had significantly increased blood serum ALT and AST compared with the control group. A comparison of different days showed significant differences with the basic group. The results showed that CuO nanoparticles increased the levels of ALT and AST enzymes in the serum, because it could be due to liver necrosis and leakage of these enzymes into the blood. Thus, these enzymes can be used as an indicator of copper oxide nanoparticle contamination.