Paper Title
Method Development and Validation of Paracetamol Drug by Rp-Hplc

Abstract
Background and aim: The manufacture of drugs from synthetic chemicals requires the control of their quality and effectiveness. The aim of the work is to contribute in the search for the use of simple, rapid and sensitize high performance liquid chromatography method for determination of paracetamol. Methods: The HPLC analysis was performed on Agilent 1260 with DAD detector at ambient temperature. A Agilent ZORBAX C18 Column (150 mm x 4.6 mm 5 μ) was utilized. A mobile phase of acetonitrile / 0.05M Phosphate Buffer, pH 4.5 (30 : 70 v/v/) was utilized at a flow rate of 1 mL/ min with UV detection at 254 nm. Standard stock solution of paracetamol was prepared by dissolving accurately weighed 10 mg of drug in 10 mL of mobile phase and filter through 0.25 μ membrane as external standard. Results: The retention time for paracetamol was found to be 1.7 min. and recovery from the formulation was 90%. The developed method was validated in terms of accuracy, precision, linearity, limit of detections, limit of quantification. Conclusion: The developed method is suitable for the identification and quantification of the paracetamol in single dosage from A. high percentage of recovery shows that the method can be successfully used on a routine basis. Keywords - RP-HPLC, Validation, Paracetamol, Acetonitrile.