Optimization of Modified Nine Media Composition for L-Asparaginase Production
This study was conducted with the aim to screen and optimize media composition to produce L-asparaginase enzyme with highest activity under optimal media composition. Selective medium was used for the production of L-asparaginase from bacteria locally isolates in Central Lab. Response surface Methodology (RSM) was employed to optimize the medium for the production of L-asparaginase at pH 8.6 and temperature of 250C. The two variables involves in this study were carbon source and nitrogen source. Primary screening resulted in Lactose and Tryptone are the best carbon and nitrogen sources with the highest enzyme activity of (2.6 IU/ml) and (2.7 IU/ml) respectively. The determination coefficient (R2for full Quadratic Model) for L-arparaginase production implied that the model is significant with high satisfactory value of R2of 90.10% which indicates that only 9.9% of total variation is not explained by the model. The optimum points were determined to be 0.189340Lactose and 8.34619 Tryptone that gave highest activity of 90.10U/ml of L-asparaginase.
Index Terms - Carbon sources, L-asparaginase, Nitrogen sources, Response surface Methodology.