Isolation and Identigication of Seed Borne Fungi from Khao Hom Mali Rice
Seed borne diseases cause significant losses in rice yield. Seed health testing to detect seed borne fungi is an important step in the management of crop diseases. This study was carried out to isolate and identify seed borne fungi associated with stored grain cultivars of Khao Hom Mali rice (Oryza sativa L. cv. KDML 105). The seeds samples were collected from the local barns that located in Lampang province, Thailand. Seed borne fungi were isolated using blotter paper and agar plate methods. A total of 7 fungi isolates were isolated from rice seed samples consisted of LPRU1, LPRU2, LPRU3, LPRU4, LPRU5, LPRU6 and LPRU7. The result from pathogenic fungal test shows that LPRU 3 had the highest effect significantly difference (P < 0.05) to incomplete growth of seedling which gave germination and complete percentage include percentage of infection at 82.33, 82.33 and 78%, respectively. This isolate was identified by morphological characterization and gene sequencing analysis. From identification conclude that LPRU3 was Bipolaris oryzae. Isolation of fungal fungi from the rice seeds indicate that they should be treated before sowing to obtain good germination and healthy crop.
Keywords: Seed borne fungi, Rice seeds, Khao Hom mali rice, Isolation, Identification