Paper Title
Anti-Inflammatory Effect of Moringaoleiferaethanolic Extract and the Chelation Ability of Phragmitesaustralis Roots Extracts

Abstract
In the present study, firstly, the activity of ethanolic extract (70%) from Moringaoleifera leaves were evaluated in vivo using xylene induced ear edema by topical application in mice as a model of acute inflammation. The oral administration of this extract at dose of 30 mg/kg or Diclofenac (anti-inflammatory standard) at dose of 50 mg/kg one hour before the induction of inflammation, showed a significant (p≤0,05) anti-inflammatory effect (86% and 73% respectively) compared to the control group considered as 100% of inflammation. Secondly, the ability of hexane (HRE), chloroform (CRE), ethyl acetate (EARE) and methanol (MRE) of Phragmitesaustralisroots extracts to chelate heavy metals, were also evaluated in vitro using ferrozine method for iron chelation and murexide method for zinc and copper chelation. The different concentrationsused (25-175µl) showed that the HRE possesses a significant (p≤0.05) capacity to chelate ferrous ions below the EDTA (standard chelator)which expresses 70% and 97% respectively. For the zinc and copper chelation ability, the results showed also that hexane extract and EDTA havethe best chelation effect towards these metals, with a percent inhibition of 55%, 56% respectively for the zinc and 47%, 88% respectively for the copper compared to the control group considered as 100%of complexation. Keywords - Inflammation, Ear edema, Moringaoleifera, Chelation, Phragmitesaustralis, ferrozine, murexide